Common Techniques

Classroom Experiments

Virtual Experiments






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Classroom Experiments

This portion of the website allows both instructors and students to view or download classroom or outreach experimental protocols.

K-12 Students
Grades K-5
Homemade Magic School Bus Book about Genetics
Crossing GloFish and Learning about colors-worksheet
GloFish colors and chromosomes-worksheet
Learning the Scientific Method by Crossing GloFish-worksheet
Learning the Scientific Method by Crossing GloFish-companion ppt
DNA in the first grade-ppt of lesson plan

Grades 6-8
Analyzing GloFish genetics with Punnett squares-worksheet
Analyzing GloFish genetics with Punnett squares-companion ppt

Grades 9-12
Mendelian inheritance of GloFish phenotypes-worksheet
Zebrafish vocabulary challenge-worksheet

Undergraduate Students
Beginner: These experiments are suitable for undergraduate students just beginning to use zebrafish in the laboratory, such as students in introductory biology courses.
Staging Experiment
Virtual Staging Experiment

Using Zebrafish to Learn Statistical Analysis and Mendelian Genetics
Samantha Lindemann, Jon Senkler, Elizabeth Auchter, Jennifer O. Liang
Zebrafish (2011) 8: 41-55.
Supplemental Materials and Figures

See Virtual Experiments for additional GloFish crosses

Cartilage staining of zebrafish embryos for high school students. Zebrafish (2009) 6: 139-143.

Set of 7 protocols adapted for use in high school biology laboratories.
Zebrafish (2009) 6: 145-160.

Oxygen consumption and metabolic rate. Zebrafish (2009) 6: 161-168.

Genetics and developmental biology intro for large intro course.
Zebrafish (2009) 6: 169-177.

Intermediate: These experiments are suitable for students who have some background in the principles of developmental biology, are familiar with using zebrafish in the laboratory, and are comfortable with common laboratory techniques such as pipetting.
Lithium Treatment to Disrupt Wnt Signaling

Cardiovascular Development using the casanova Mutant
Virtual casanova Experiment

Advanced: These experiments are suitable for students who have a background in developmental biology, are familiar with using zebrafish in the laboratory, are comfortable with advanced developmental biology terminology, and have significant experience working in the laboratory.
Microinjection of Zebrafish Embryos

Analysis of zebrafish motor behavior. Zebrafish (2009) 6: 179-185.

Differential gene expression. Zebrafish (2009) 6: 187-199.

Using zebrafish in a biomedical engineering design course.
Zebrafish (2009) 6: 201-213.

Zebrafish fin regeneration

Factors promoting increased rate of tissue regeneration: The zebrafish fin as a tool for examining tissue engineering design concepts. Zebrafish (2012) 9: 207-219.

Activation of Wnt signaling using lithium chloride: Inquiry-based undergraduate laboratory excercises. Zebrafish (2012) 9: 220-225.

Learning the scientific method using GloFish. Zebrafish (2012) 9: 226-241.

Outreach: These experiments are designed for outreach to local schools or to the community.

Community outreach by the National Institute for Environmental Studies in Japan. Zebrafish (2009) 6: 127-132.

Bioeyes program-outreach to K-12. Zebrafish (2009) 6: 133-138.

Fish Cam: An online tool for introducing shoaling behavior to the classroom. Zebrafish (2012) 9: 242-245.

Improvement in Student Science Proficiency Through InSciEd Out. Zebrafish (2012) 9: 155-168.