This portion of the website allows both instructors and students to view or download classroom or outreach experimental protocols.
Homemade Magic School Bus Book about Genetics
Crossing GloFish and Learning about colors-worksheet
GloFish colors and chromosomes-worksheet
Learning the Scientific Method by Crossing GloFish-worksheet
Learning the Scientific Method by Crossing GloFish-companion ppt
DNA in the first grade-ppt of lesson plan
Analyzing GloFish genetics with Punnett squares-worksheet
Analyzing GloFish genetics with Punnett squares-companion ppt
Mendelian inheritance of GloFish phenotypes-worksheet
Zebrafish vocabulary challenge-worksheet
Beginner: These experiments are suitable for undergraduate students just beginning to use zebrafish in the laboratory, such as students in introductory biology courses.
Virtual Staging Experiment
Using Zebrafish to Learn Statistical Analysis and Mendelian Genetics
Samantha Lindemann, Jon Senkler, Elizabeth Auchter, Jennifer O. Liang
Zebrafish (2011) 8: 41-55.
Supplemental Materials and Figures
Dihybrid cross example 1 (GloPFP and golden):
-Worksheet (with answers)
-Data figure for cross
Dihybrid cross example 2 (GloBFP and GloRFP):
-Introduction and worksheet
-Ppt of lesson plan
Cartilage staining of zebrafish embryos for high school students. Zebrafish (2009) 6: 139-143.
Set of 7 protocols adapted for use in high school biology laboratories.
Zebrafish (2009) 6: 145-160.
Oxygen consumption and metabolic rate. Zebrafish (2009) 6: 161-168.
Genetics and developmental biology intro for large intro course.
Zebrafish (2009) 6: 169-177.
Intermediate: These experiments are suitable for students who have some background in the principles of developmental biology, are familiar with using zebrafish in the laboratory, and are comfortable with common laboratory techniques such as pipetting.
Lithium Treatment to Disrupt Wnt Signaling
Cardiovascular Development using the casanova Mutant
Virtual casanova Experiment
Advanced: These experiments are suitable for students who have a background in developmental biology, are familiar with using zebrafish in the laboratory, are comfortable with advanced developmental biology terminology, and have significant experience working in the laboratory.
Microinjection of Zebrafish Embryos
Analysis of zebrafish motor behavior. Zebrafish (2009) 6: 179-185.
Differential gene expression. Zebrafish (2009) 6: 187-199.
Using zebrafish in a biomedical engineering design course.
Zebrafish (2009) 6: 201-213.
Zebrafish fin regeneration
Factors promoting increased rate of tissue regeneration: The zebrafish fin as a tool for examining tissue engineering design concepts. Zebrafish (2012) 9: 207-219. http://online.liebertpub.com/doi/pdfplus/10.1089/zeb.2012.0741
Activation of Wnt signaling using lithium chloride: Inquiry-based undergraduate laboratory excercises. Zebrafish (2012) 9: 220-225.
Learning the scientific method using GloFish. Zebrafish (2012) 9: 226-241.
Outreach: These experiments are designed for outreach to local schools or to the community.
Community outreach by the National Institute for Environmental Studies in Japan. Zebrafish (2009) 6: 127-132.
Bioeyes program-outreach to K-12. Zebrafish (2009) 6: 133-138. http://www.liebertonline.com/doi/pdfplus/10.1089/zeb.2008.0568
Fish Cam: An online tool for introducing shoaling behavior to the classroom. Zebrafish (2012) 9: 242-245. http://online.liebertpub.com/doi/pdfplus/10.1089/zeb.2012.0755